Enviado por Secretaria em qui, 12/11/2015 – 15:56
Oferecimento extra de Disciplina: RBP-5757 – Aspectos Moleculares da Biologia de Parasitas Protozoários
A Disciplina RBP-5757 – Aspectos Moleculares da Biologia de Parasitas Protozoários será ofertada no período de 23/11/2015 a 27/11/2015 das 09:00 às 12:00 hrs e das 14:00 às 18:00 hrs todos os dias. Por favor, quem possuir interesse em cursar esta disciplina entregar na Secretaria do Programa o formulário anexo devidamente preenchido, impreterivelmente até o dia 18/11/2015 às 11:30 hrs.
Course outline: Genetics of Kinetoplastid Parasites
Course Organizer: Dr. Pegine Walrad
Programa da disciplina oferecido nesse período
This course is designed to provide the students with an overall perspective on current genetics in the field of protozoan parasites. It will review traditional eukaryotic gene regulation and highlight significant distinctions in parasites. It will examine the history of investigations that have led to important innovations in the research field and discuss the currently available and much-needed genetic tools in the different protozoan species. Participants will get hands-on experience with planning, optimising and troubleshooting protocols for distinct parasite assays. Graduates will establish confidence and competence in parasite genetics knowledge and skills.
| Monday 23/11/15 |
Tuesday 24/11/15 |
Wednesday 25/11/15 |
Thursday 26/11/15 |
Friday 27/11/15 |
| Transcriptional control: Important parasite distinctions |
Post-transcriptional control: mechanisms in protozoans |
Post- translational control: mechanisms in protozoans |
Genetic Tools and significant advances |
Troubleshooting, Course Summary |
| Practical: DNA isolation, Diagnostic PCRs |
Practical: RNA isolation, RTPCR, PCRs |
Practical: Phosphorylation, Acetylation or Methylation assay |
Practical: Transfections Electroporation versus Nucleofection |
Practical: Vector design, Parasite-specific protocol modifications |